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コラーゲン足場を用いた立体組織灌流培養システムの開発に関する に関する論文がInternational Journal of Molecular Sciences誌に掲載されました

今城哉裕博士(ポスト・ドクター)の論文です。

“Perfusable System Using Porous Collagen Gel Scaffold Actively Provides Fresh Culture Media to a Cultured 3D Tissue”

IMASHIRO Chikahiro†,  YAMASAKI Kai, TANAKA Ryu-ichiro, TOBE Yusuke, SAKAGUCHI Katsuhisa*, SHIMIZU Tatsuya

International Journal of Molecular Sciences, 22(13), 6780(2021)

doi:10.3390/ijms22136780

Abstract

Culturing three-dimensional (3D) tissues with an appropriate microenvironment is a critical and fundamental technology in broad areas of cutting-edge bioengineering research. In addition, many technologies have engineered tissue functions. However, an effective system for transporting nutrients, waste, or oxygen to affect the functions of cell tissues has not been reported. In this study, we introduce a novel system that employs diffusion and convection to enhance transportation. To demonstrate the concept of the proposed system, three layers of normal human dermal fibroblast cell sheets are used as a model tissue, which is cultured on a general dish or porous collagen scaffold with perfusable channels for three days with and without the perfusion of culture media in the scaffold. The results show that the viability of the cell tissue was improved by the developed system. Furthermore, glucose consumption, lactate production, and oxygen transport to the tissues were increased, which might improve the viability of tissues. However, mechanical stress in the proposed system did not cause damage or unintentional functional changes in the cultured tissue. We believe that the introduced culturing system potentially suggests a novel standard for 3D cell cultures.

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