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ブロック共重合体コーティングによるセルカルチャーインサートへの温度応答性付与に関する論文がJournal of Materials Chemistry Bに掲載されました。

中山正道 講師の論文です。

“Water stable nanocoatings of poly(N-isopropylacrylamide)-based block copolymers on culture insert membranes for temperature-controlled cell”

NAKAYAMA Masamichi†*, TOYOSHIMA Yuki, CHINEN Hiroshi, KIKUCHI Akihiko, YAMATO Masayuki, OKANO Teruo

Journal of Materials Chemistry B, 2020, in press

doi:10.1039/D0TB01113D (Paper)

Abstract

This study demonstrated the spin-coating of functional diblock copolymers to develop smart culture inserts for thermoresponsive cell adhesion/detachment control. One part of the block components, the poly(n-butyl methacrylate) block, strongly supported the water stable surface-immobilization of the thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) block, regardless of temperature. The chain length of the PNIPAAm blocks was varied to regulate thermal surface functions. Immobilized PNIPAAm concentrations became larger with increasing chain length(1.0–1.6 μg cm⁻²)and the thicknesses of individual layers were relatively comparable at 10-odd nanometers. A nanothin coating scarcely inhibited the permeability of the original porous membrane. When human fibroblasts were cultured on each surface at 37 °C, the efficiencies of cell adhesion and proliferation decreased with longer PNIPAAm chains. Meanwhile, by reducing the temperature to 20 °C, longer PNIPAAm chains promoted cell detachment owing to the significant thermoresponsive alteration of cell-surface affinity. Consequently, we successfully produced a favorable cell sheet by choosing an appropriate PNIPAAm length for block copolymers.

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