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細胞シート工学を利用した細胞マイクロ流体デバイスに関するに関する論文がCyborg and Bionic Systems誌に掲載されました

坂口勝久 非常勤講師(早稲田大学)の論文です。

“Cell-Based Microfluidic Device Utilizing Cell Sheet Technology”

SAKAGUCHI Katsuhisa†*, AKIMOTO Kei, TAKAIRA Masanori, , TANAKA Ryu-ichiro,  SHIMIZU Tatsuya, UMEZU Shinjiro*

Biofabrication, Accepted Manuscript online 6 September 2021

doi:10.34133/2022/9758187

Abstract

The development of microelectromechanical systems has resulted in the rapid development of polydimethylpolysiloxane (PDMS) microfluidic devices for drug screening models. Various cell functions, such as the response of endothelial cells to fluids, have been elucidated using microfluidic devices. Additionally, organ-on-a-chip systems that include organs that are important for biological circulation, such as the heart, liver, pancreas, kidneys, and brain, have been developed. These organs realize the biological circulation system in a manner that cannot be reproduced by artificial organs; however, the flow channels between the organs are often artificially created by PDMS. In this study, we developed a microfluidic device consisting only of cells, by combining cell sheet technology with microtitanium wires. Microwires were placed between stacked fibroblast cell sheets, and the cell sheets adhered to each other, after which the microwires were removed leaving a luminal structure with a size approximately equal to the arteriolar size. The lumen structure was constructed using wires with diameters of 50, 100, 150, and 200 μm, which were approximations of the arteriole diameters. Furthermore, using a perfusion device, we successfully perfused the luminal structure created inside the cell sheets. The results revealed that a culture solution can be supplied to a cell sheet with a very high cell density. The biofabrication technology proposed in this study can contribute to the development of organ-on-a-chip systems.

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