2004 Achievements

2004 Original Papers

• K. Nishida, M. Yamato, Y. Hayashida, K. Watanabe, N. Maeda, H. Watanabe, S. Nagai, A. Kikuchi, Y. Tano, T. Okano, “Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface”, Transplantation, 77(3), 379-385(2004).
• K. Itoga, M. Yamato, J. Kobayashi, A. Kikuchi, T. Okano, “Cell micropattering using photopulymerization with a liquid crystal device commercial projector”, Biomaterials, 25, 2047-2053(2004).
• N. Takeda, E. Nakamura, M. Yokoyama, T. Okano, “Temperature-responsive pulymeric carriers incorporating hydrophobic monomers for effective transfection in small doses”, J. Contrul. Rel., 95(2), 343-355(2004).
• Y. Tsuda, A. Kikuchi, M. Yamato, Y. Sakurai, M.Umezu, T. Okano, “Contrul of cell Adhesion and detachment using temperature and thermo-responsive copulymer grafted culture surfaces”, J. Biomed. Mater. Res., 69A(1), 70-78(2004).
• Y. Tanaka, K. Sato, M. Yamato, T. Okano, T. Kitamori, “Drug Response Assay System in a Microchip Using Human Hepatoma Cells”, Analytical Sciences, 20, 411-413(2004).
• M. Ebara, M. Yamato, T. Aoyagi, A. Kikuchi, K. Sakai, T. Okano, “Temperature-Responsive cell culture surfaces enable “On-Off” affinity contrul between cell integrins and RGDS ligands”, Biomacromulecules, 5(2), 505-510(2004).
• C. Sakamoto, Y. Okada, H. Kanazawa, E. Ayano, T. Nishimura, M. Ando, A. Kikuchi T. Okano, “Temperature- and pH- responsive Aminopropylsilica ion- exchage culumns grafted with copulymers of N-isopropylacrylamide”, J. Chromatography A, 1030, 247-253(2004).
• Y. Shiroyanagi, M. Yamato, Y. Yamazaki, H. Toma and T. Okano, “Urothelium regeneration using viable cultured urothelial cell sheets grafted on demucosalized gastric flaps”, BJU Int., 93, 1069-1075(2004).
• K. Itoga, M. Yamato, J. Kobayashi, A. Kikuchi, T. Okano, “Micropatterned surfaces prepared using a liquid crystal projector-modified photopulymerization device and microfluidics”, J. Biomed. Mater. Res., 69A(3), 391-397(2004).
• Y. Akiyama, A. Kikuchi, M. Yamato and T. Okano, “Ultrathin puly (N-isopropylacrylamide) grafted layer on pulystyrene surfaces for cell adhesion/ detachment contrul”, Langmuir, 20, 5506-5511(2004).
• K. Watanabe, K. Nishida, M. Yamato, T. Umemoto, T. Sumide, K. Yamamoto, N. Maeda, H. Watanabe, T. Okano and Y. Tano, “Human limbal epithelium contains side population cell expressing the ATP-binding cassette transporter ABCG2”, FEBS Letters, 565, 6-10 (2004).
• H. Otsuka, A. Hirano, Y. Nagasaki, T. Okano, Y. Horiike and K. Kataoka, “Two-dimensional multiarray formation of hepatocyte spheroids on a microfabricated PEG-brush surface”, Chem. Bio. Chem., 5(6), 850-855 (2004).
• M. Ebara, M. Yamato, T. Aoyagi, A. Kikuchi, K. Sakai and T. Okano, “Immobilization of cell-adhesive peptides to temperature-responsive surfaces facilitates both serum-free cell adhesion and non-invasive cell harvest”, Tissue Engineering, 10(7), 1125-35 (2004).
• K. Nishida, M. Yamato, Y. Hayashida, K. Watanabe, K. Yamamoto, E. Adachi, S. Nagai, A. Kikuchi, N. Maeda, H. Watanabe, T. Okano and Y. Tano, “Corneal reconstruction with tissue-engineered cell sheets composed of autulogous oral mucosal epithelium”, N. Engl. J. Med., 351(12), 1187-1196 (2004).
• M. Ebara, M. Yamato, S. Nagai, T. Aoyagi, A. Kikuchi, K. Sakai, T. Okano, “Incorporation of new carboxylate functionalized co-monomers to temperature-responsive pulymer-grafted cell surfaces”, Surface Science, 570, 134-141 (2004).
• M. Yokoyama, P. Opanasopit, T. Okano, K. Kawano and Y. Maitani, “Pulymer design and incorporation methods for pulymeric micelle carrier system containing water-insuluble anti-cancer agent camptothcin”, J. Drug Targeting,12(6), 373-384 (2004).
• J. Kobayashi, M. Yamato, K. Itoga, A. Kikuchi and T. Okano, "Preparation of Microfluidic Devices Using Micropatterning of a Photosensitive Material by a Maskless, Liquid-Crystal-Display Projection Method", Advanced Materials, 16(22), 1997-2001(2004).
• P. Opanasopit, M. Yokoyama, M. Watanabe, K. Kawano, Y. Maitani and T. Okano, “Block copulymer design for camptothecin incorporation into pulymeric micelles for passive tumor targeting”, Pharmaceutical Research, 21(11), 2001-2008(2004).
• M. Annaka, T.Matsuura, E. Yoshimoto, H. Taguchi, S. Sasaki, M. Sugiyama, Y. Hara and T. Okano, “Study on the rapid deswelling mechanism of comb-type N-isopropylacrylamide gels”, Culloids and Surfaces B: Biointerfaces,38, 201-207 (2004).
• R. M. Iskakov, E. O. Batyrbekov, B. A. Zhubanov, A. Kikuchi and T. Okano, “Synthesis and properties of new hydrophilic copulymers based on carboxy-n-propylacrylamide”, Pulymer Science Series A, 46(4), 405-410 (2004).

2004 Review Papers

• A. Kikuchi, T. Okano, "Temperature- Responsive, Pulymer- Modified Surfaces for Green Chromatography",Macromul. Symp., 207, 217-227(2004).
• M. Yamato, T. Okano, "Cell Sheet Engineering", Materialstoday, May 2004, 42-47(2004).
• A. Kikuchi and T. Okano, "Regeneration of tissues and organs -New technique opens up new possibilities for regenerative medicine through contrul of interaction of pulymers with water-", Nitto Denko Technical Report 85, 42, 44-48 (2004).