Cell Sheet Engineering
Temperature-responsive cell culture vessels
Our core technology features a nano-bio interface surface, which consists of a temperature-responsive polymer immobilized to the cell culture surface at nanometer-scale thickness. The surface shows similar hydrophobicity as normal polystyrene tissue culture dishes at 37°C. When the temperature is reduced below 32°C, the surface rapidly hydrates and becomes hydrophilic. (Figure 1) Therefore, by only reducing temperature viable cultured cells and intact deposited ECM (extracellular matrix) are recovered as a cell sheet from the temperature-responsive culture dish surface with no requirement of digestive enzymes or denaturing treatments. (Figure 2)
Feature of temperature-responsive polymer
Schematic diagram of cell sheet isolation from
temperature-responsive cell culture surface
Cell sheet engineering as a platform technology for regenerative medicine
Cell sheets can be prepared using cells from various biological tissues and organs. These can be used alone as tissue for transplantation, or used in combination with sheets composed of other cells, and can be used either singly, or in stacked arrangements of multiple cell sheets.